![]() I., Veeraraghavan, J., Polaczek, P., Campbell, J. RPA governs endonuclease switching during processing of Okazaki fragments in eukaryotes. Distribution of functions between FEN1 and DNA2. Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro. Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40. An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase. Polymerase dynamics at the eukaryotic DNA replication fork. ![]() The replication fork, core histone segregation and terminal structures. Structure of replicating simian virus 40 minichromosomes. Making copies of chromatin: the challenge of nucleosomal organization and epigenetic information. Our studies represent the first high-resolution analysis-to our knowledge-of eukaryotic Okazaki fragments in vivo, and reveal the interconnection between lagging-strand synthesis and chromatin assembly.Ĭorpet, A. Disrupting chromatin assembly or lagging-strand polymerase processivity affects both the size and the distribution of Okazaki fragments, suggesting a role for nascent chromatin, assembled immediately after the passage of the replication fork, in the termination of Okazaki fragment synthesis. Using deep sequencing, we demonstrate that ligation junctions preferentially occur near nucleosome midpoints rather than in internucleosomal linker regions. Here we show that ligation-competent Okazaki fragments in Saccharomyces cerevisiae are sized according to the nucleosome repeat. Eukaryotic Okazaki fragments remain poorly characterized and, because nucleosomes are rapidly deposited on nascent DNA, Okazaki fragment processing and nucleosome assembly potentially affect one another. Fifty per cent of the genome is discontinuously replicated on the lagging strand as Okazaki fragments.
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